The Mass Spectrometry society of Japan - The 68th Annual Conference on Mass Spectrometry, Japan
Japanese

Abstract

Table of contents

Timetable

Plenary Lectures

Oral Sessions (Day1, Day2, Day3)

Poster Presentations (Day1, Day2, Day3)

Workshops

Luncheon Seminars (Day1, Day2, Day3)

Poster Presentations

Day 1, May 11（Mon.）　　Poster (1008/09)

1P-36（1C-O3-1530）　　PDF

MALDI-MS Analysis of Antitumor Antibiotics with Thiol containing Matrix

(Yokohama City Univ.)
oHajime Yoshikawa, Mitsuo Takayama

The enediyne natural products are effective drugs for cancer or leukemia owing to their strong DNA cleavage activity. Bioreduction triggers cyclization of their enediyne portion producing a 1,4-dehydrobenzene diradical species, and it abstracts two hydrogens from deoxyribose. MALDI mass spectrum of Esperamicin-A1, one of the first isolated enediyne antibiotics, with thiol containing matrix (2,5-Diamino-1,4-benzenedithiol) gave peaks of the cleavage of the trisulfide with thiols (m/z 1248) and cyclized analyte (m/z 1250, Esperamicin-Z). However, other matrixes including 1,4-Benzenedithiol gave no peaks of Esperamicin-Z.