Poster Presentations
- Day 1, May 11（Mon.） Poster (1008/09)
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1P-35 PDF
Analysis of Hydrophilic Compounds by Triple Quadrupole LC-MS—A Detailed Investigation of When to Use HILIC Versus a Reversed-Phase LC Column
We examined when to use hydrophilic interaction LC (HILIC) and when to use reversed-phase LC (RPLC) columns for analyzing difficult-to-retain small polar and ionized molecules. An Agilent 1290 Infinity II LC with an Agilent Ultivo LC/TQ was used in this experiment. All columns were used in a 2.1 × 100 mm format. Amino acids and water solubility vitamins were selected as analyte compounds. The primary difference in mobile phase between HILIC and RPLC is solvent elution strength. For RPLC, acetonitrile is the strong eluting solvent. However, for HILIC, water is the strong eluting solvent. Careful consideration should be given to which type of method will meet separation requirements, will work best with the sample, and which types of detection are feasible. Moreover, these solvent conditions also affect the MS sensibility because of their vapor temperature differences. We demonstrated here the impact of analytes/samples, mobile phases, modes of detection, and other method suitability requirements (retention, resolution, sensitivity, and peak shape). These results indicated that choosing between HILIC and RPLC represents a balancing act that benefits from careful consideration of chromatographic separation requirements, analyte polarity and solubility, the nature of the sample solvent, and available detection modalities.