Abstract

Poster Presentations

Day 1, May 11（Mon.）　　Poster (1008/09)

1P-34　　PDF

A Quantitative Targeted-SIM Assay for Cancer Biomarker using Nano-liquid Chromatography Coupled with High-resolution Accurate Mass Spectrometer

(¹Daiichi Univ. of Pharmacy, ²NISSHA, ³St. Marianna Univ. School of Medicine)
^oKiyonaga Fujii^1,3, Yuka Miyata², Ikuya Takahashi², Hirotaka Koizumi³, Masayuki Takagi³, Toshihide Nishimura³, Haruhiko Nakamura³, Hisashi Saji³

In targeted proteomics, MS-based assay provides a quantitative, sensitive, and reproducible approach for analysis of proteins in any biological samples. Selected/multiple reaction monitoring (SRM/MRM) using triple-quadrupole instrument is the most widely used for the quantification of specific proteins/peptides, although it is still challenging to quantitate low abundant proteins/peptides in complex samples from clinical specimens. In this study, we investigated the establishment of targeted selected ion monitoring (t-SIM) methodology using nano-liquid chromatography system coupled with a Q-Exactive high-resolution accurate mass spectrometer. Furthermore, we applied the well-established t-SIM platform to relative quantitation of neuron-specific enolase (NSE, gamma-enolase), one of small-cell lung cancer (SCLC) biomarkers, in cancerous cells laser-microdissected from formalin-fixed paraffin-embedded tissue samples from SCLC patients.