Abstract

Poster Presentations

Day 1, May 11（Mon.）　　Poster (1008/09)

1P-28　　PDF

Development of a method for analysis of O-linked glycopeptides using sialic acid linkage-specific derivatization

(¹TMIG, ²Shimadzu)
^oHiroki Tsumoto¹, Takashi Nishikaze², Sadanori Skiya², Keitaro Umezawa¹, Kyojiro Kawakami¹, Shinichi Iwamoto², Koichi Tanaka², Yuri Miura¹, Tamao Endo¹

We developed a method for linkage-specific sialic acid analysis of sialylated O-glycopeptides by a combination of sialic acid linkage-specific alkylamidation (SALSA) and nanoLC-ESI-MS/MS. This method could be used for analysis of protein sialylation with determination of sialic acid linkage.