The Mass Spectrometry society of Japan - The 68th Annual Conference on Mass Spectrometry, Japan

Abstract

Poster Presentations

Day 1, May 11(Mon.)  Poster (1008/09)

A novel method for obtaining peptide sequence information using AP/MALDI-MS

(1Hiroshima Univ., 2Shimadzu)
oManaho Yamaguchi1, Koichi Tanaka2, Takushi Yamamoto2, Shunsuke Izumi1

Generally, MALDI is considered to be a soft ionization method capable of obtaining molecular weight information of a sample with minimum fragmentation. It is thought that less fragmentation occurs in Atmospheric Pressure (AP) MALDI-MS. However, a lot of a-, b- and d-series ions were generated from Angiotensin II (DRVYIHPF) mixed with a small amount of CHCA matrix under less than 10m laser spot size conditions using iMScope, an AP-MALDI instrument.