The Mass Spectrometry society of Japan - The 68th Annual Conference on Mass Spectrometry, Japan
Japanese

Abstract

Table of contents

Timetable

Plenary Lectures

Oral Sessions (Day1, Day2, Day3)

Poster Presentations (Day1, Day2, Day3)

Workshops

Luncheon Seminars (Day1, Day2, Day3)

Poster Presentations

Day 1, May 11（Mon.）　　Poster (1008/09)

1P-21　　PDF

Inter laboratory study of human plasma metabolomics by GC/MS with a calibration-curve-locking database (CCLD)

(1Agilent, 2Nagoya Univ., 3Tokyo Univ. Agr., 4FRA, 5Kyushu Univ.)
oAya Anegawa1, Yumi Aizawa3, Jun Ueyama2, Yoshimasa Tsujii3, Takeshi Hano4, Kosuke Hata5, Toshiyuki Yamashita5, Yoshihiro Izumi5, Takeshi Bamba5

In this study, we performed cross-laboratory validation of gas chromatography/mass spectrometry (GC/MS) with a calibration-curve-locking database (CCLD) to integrate metabolome data obtained at different facilities. The GC/MS-based metabolomic data was compared at five different laboratories using identical plasma samples, pretreatment method, and GC/MS analysis. Results indicated that the concentration of hydrophilic metabolites in human plasma NIST SRM1950 is almost consistent with the certificated values. Thus, these studies suggested the possibility of semi-quantification screening using CCLD without requiring calibration curve.