The Mass Spectrometry society of Japan - The 68th Annual Conference on Mass Spectrometry, Japan
Japanese

Abstract

Table of contents

Timetable

Plenary Lectures

Oral Sessions (Day1, Day2, Day3)

Poster Presentations (Day1, Day2, Day3)

Workshops

Luncheon Seminars (Day1, Day2, Day3)

Poster Presentations

Day 1, May 11（Mon.）　　Poster (1008/09)

1P-15　　PDF

OPIG-DC Voltage Resolved Proton Transfer Reactions of Cytochrome c Multi-charged Ions

(Yokohama City Univ.)
oYuto Yonebayashi, Satoko Kanamori, Saki Kitamura, Takaaki Iyama, Wataru Kadota, Julie Morishita, Shinji Nonose

In vivo, it is difficult to examine the structure and function of biomolecules because there are interactions with water molecules in addition to interactions between biomolecules. The purpose of this study was to investigate the properties of biomolecules in the absence of interaction with other molecules by introducing them into a vacuum. In this study, a self-made tandem mass spectrometer consisting of a quadrupole mass spectrometer (QMASS) and a flight mass spectrometer (TOF-MS) using an electrospray ionization source was used. Using the ionic strength value of the obtained mass spectrum, the reaction rate constantk of the proton transfer reaction was determined. Then, the dependence of proton transfer reaction on DC voltage of octapole ion guide (VDC) was examined. As a result of the experiment, it was found that the reaction rate constant k may increase or decrease as the VDC increases.