Abstract

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Poster Presentations

Day 1, May 11（Mon.）　　Poster (1008/09)

1P-07　　PDF

Time-, Temperature-, and OPIG-DC Voltage- Resolved Proton Transfer Reactions of Lysozyme Ions

(Yokohama City Univ.)
^oTakaaki Iyama, Satoko Kanamori, Saki Kitamura, Wataru Kadota, Julie Morishita, Yuto Yonebayashi, Shinji Nonose

Time-, and OPIG-DC voltage-resolved proton transfer reactions of protonated lysozyme (Lys) ions, [M + zH]^z+, to gaseous molecules were examined in the gas phase. Absolute reaction rate constants for proton transfer were determined from intensities of precursor and product ions in the mass spectra. With changes of reaction temperature and OPIG-DC voltage, remarkable change was observed for distribution of product ions and reaction rate constants. In this study, we examined at 296 and 433 K. When [M + 8H]⁸⁺was selected, the OPIG-DC voltage increased at 296K and the proton transfer reactions from [M + 7H]⁷⁺ to [M + 6H]⁶⁺ was prompted. But at 433K didn’t prompted. So by changing OPIG-DC voltage, we investigated the reactivity of [M + 7H]⁷⁺ which was selected from [M + 8H]⁸⁺at various reaction times and reaction temperatures. As the result, the reactivity of [M + 7H]⁷⁺changed at a certain reaction times and reaction temperatures. When OPIG-DC was increased at 296K, Lys was subjected to severe thermal motion to suppress the proton transfer reaction. It is consider that this is because the stable structure of Lys is changed and proton transfer is suppressed.