The Mass Spectrometry society of Japan - The 68th Annual Conference on Mass Spectrometry, Japan
Japanese

Abstract

Table of contents

Timetable

Plenary Lectures

Oral Sessions (Day1, Day2, Day3)

Poster Presentations (Day1, Day2, Day3)

Workshops

Luncheon Seminars (Day1, Day2, Day3)

Oral Sessions

Day 1, May 11（Mon.）　15:30-15:50　Room A (Main Hall)

1A-O1-1530　　PDF

Native mass spectrometry of proteins originated from living cells

(1Yokohama City Univ., 2Yokohama City Univ.)
Shunsuke Arai1, Waka Sakamoto2, Kotaro Takano1, Tsuyoshi Konuma1, oSatoko Akashi1

Native mass spectrometry (Native MS) with nanoESI enables observation of intact ions of protein complexes with a small amount of sample. It has long been considered that the samples for native MS are required to purify as much as possible and to prepare in volatile solutions, such as aqueous ammonium acetate. In the present study, we examined native MS to observe intact ions of protein complexes without any purification procedures from the crude cell lysate. Optimization of the experimental conditions allowed detection of stable protein ions. Furthermore, intact hemoglobin ions could be observed for the crude red blood cells, without purification with any chromatography.