3P-51 PDF
Hydrogen Attachment/Abstraction Dissociation (HAD)を用いた低価数ペプチドのLC-ESI-MS/MS分析
Hydrogen attachment/abstraction dissociation tandem mass spectrometry (HAD MS/MS) was subjected to tryptic peptides using a LCMS-IT-TOF (Shimadzu, Japan). The HAD spectra of tryptic bovine serum albumin (BSA) digest were obtained by a data-dependent acquisition based on the precursor ion intensity. As a preliminary result of the Mascot database search, over 20 unique peptides were identified with false discovery rate less than 1% independently for HAD MS/MS and CID MS/MS. Normalizing the frequency of the matched fragment ion types, the frequency of occurrence of N-terminal fragment ions (c-type ions) was equivalent to that of C-terminal fragment ions (z-type ions) for HAD MS/MS of 2+ tryptic peptides. The appearance of complementary ion pairs from a particular bond cleavage (c-/z- ion pair) provides a straightforward interpretation of HAD MS/MS spectra.