演題概要

ポスター発表

第3日　5月19日（金）　　P会場（多目的ホール）

3P-25　　PDF

分離モード並列化による親水性代謝物の包括的メタボローム分析

(¹九大生医研・ ²サーモフィッシャー)
^o和泉自泰¹・ 鈴木隆弘²・ 高橋政友¹・ 中谷航太¹・ 押川清孝¹・ 木村元一²・ 高原健太郎²・ 松本雅記¹・ 馬場健史¹

Essential metabolic pathways to maintain biological activities include glycolysis, pentose phosphate pathway, tricarboxylic acid cycle, amino acid, and nucleic acid metabolism. Almost all intermediates of these metabolic pathways are sugar phosphates, organic acids, amino acids, and nucleic acids. Therefore, a profiling for these hydrophilic metabolites provides valuable insight towards understanding the different biochemical profiles of a biosystem. In this study, we established a high-accuracy and robust method that enables the comprehensive analysis of polar metabolites by a combination of ion chromatography quadrupole Orbitrap mass spectrometry (IC/Q Exactive), and pentafluorophenyl propyl (PFPP) based liquid chromatography/Q Exactive (PFPP-LC/Q Exactive), and an automated library-based compounds identification. The developed analytical system is a potentially useful tool in the field of life science, medical, and pharmaceutical research.