演題概要

ポスター発表

第3日　5月19日（金）　　P会場（多目的ホール）

3P-18　　PDF

心血管疾患病理解析のためのイメージング質量分析法

(¹ブルカーダルトニクス・ ²同志社大生命医・ ³Georges Pompidou European Hospital・ ⁴国循セ)
^o韮澤崇¹・ 難波寛子²・ 寺田恵²・ 工藤寿治¹・ 宮坂知宏²・ 角田伸人²・ Patrick, Bruneval³・ 植田初江⁴・ 池川雅哉²

Here we have developed a novel protocol for imaging mass spectrometry of human cardiovascular tissues. By using formalin fixed paraffin embedded biopsied samples, we have succeeded in visualization of peptides and proteins of the tissues with on tissue trypsinization. Comprehensive visualization of abnormal deposition of various peptide species was enabled with multivariate analysis. Furthermore, it is promising that this technique can identify unknown factor(s) regulating pathogenesis of the disease yet to be defined. Through these advancements, interinstitutional comparison must be the next step as well as antibody based validation for each biomarker candidate.