演題概要

ポスター発表

第3日　5月19日（金）　　P会場（多目的ホール）

3P-12　　PDF

スプライシングを制御するRNA結合蛋白質の解析法の構築

(¹神戸薬大・ ²神大・ ³神戸学院大)
^o竹内敦子¹・ 寳田徹¹・ 吉野健一²・ 松尾雅文³

Splicing regulatory factors participate the regulation of splice site usage. To identify these factors bound to the targeted exon, we designed in vitro binding assay. The RNA probe was incubated with the cell nuclear extract. The separated band in PAGE, which contains the RNA-protein complex, was in-gel digested. This peptide mixture was applied to LC-MS/MS to identify the binding protein. To confirm the utility of this method, we designed an RNA probe consisting of the insulin receptor exon 11. We found that hnRNPA1 forms the complex with the exon11 sequence.