3C-O2-1525 PDF
糖鎖MS/MSフラグメンテーションにおけるN-アセチル基の影響
We analyzed six different sequences of liner glucohexaoses having one N-acetyl hexosamine (HexNAc) by MALDI-TOF/TOF MS. All sugar chains were labeled by pyridylamination (PA). The all MS/MS fragment ions from [M+H]+ precursor ion had the reducing end sugar substituted by PA group, and those fragment ions were sequentially generated by charge-remote fragmentation. These fragment ions were useful for the sugar chain sequence because they indicated the position of the HexNAc residue. The fragment ions from the sodium adducted molecule [M+Na]+ were much more complex than those from [M+H]+. Those fragment ions were generated with or without the PA reducing end residue. Since almost of all fragment ions had HexNAc residue, the sodium atom localized at HexNAc reside.