演題概要

オーラルセッション

第3日　5月19日（金）　10:10～10:30　C会場（101）

3C-O1-1010　　PDF

MIK-MS（Molecular Interaction & Kinetics Mass Spectrometry)を用いたタンパク質-低分子化合物のMS Sensorgram

(¹日本写真印刷（株）・ ²バイオシス・ ³聖マリアンナ医科大学・ ⁴Silicon Kinetics Inc.・ ⁵東大工・東大創薬・ ⁶東京大学アイソトープ総合センター・先端化学技術研究センター)
^o小尾奈緒子¹・ 福田哲也²・ 中山登²・ Ervin, John⁴・ 板東泰彦²・ 西村俊秀^2,3・ 染原俊朗¹・ 長門石曉⁵・ 津本浩平⁵・ 川村猛⁶

Molecular Interaction & Kinetics Mass Spectrometry (MIK-MS) would be a powerful tool to screen drug candidates by integrating MS with nano Pore Optical Interferometry (nPOI). nPOI can measure the association and dissociation rates of biomolecules without labels, like Surface Plasmon Resonance. Since nPOI has a much larger ligand capture capacity, it can be used to directly detect analytes using a nano ESI LC-MS backend, thereby confirming the binding and the analyte. Here we will report that obtained MS sensorgram and calculated Koff ranking of the intermolecular interaction between Carbonic Anhydrase II and multiple small molecules using MIK-MS.