2C-O1-0910 PDF
iMPAQT: A platform for large-scale targeted proteomics based on an in vitro human proteome
We have now developed a new targeted proteomics platform—in vitro proteome–assisted MRM for protein absolute quantification (iMPAQT)—using >18,000 human recombinant proteins for genome-wide protein absolute quantification (Matsumoto et al., Nature Methods, 2017). This novel platform comprises experimentally confirmed MRM assays of mass tag labeled peptides to allow the rapid and relatively easy measurement of the absolute abundance of predefined protein sets. We applied iMPAQT to delineate the quantitative metabolic landscape of normal and transformed human fibroblasts. Oncogenic transformation gave rise to relatively small but global changes in metabolic pathways that account for aerobic glycolysis (Warburg effect) and increased rates of macromolecule synthesis. The iMPAQT should greatly facilitate quantitative biology based on protein abundance.