1P-42 PDF
創薬ターゲット化合物スクリーニングを目指した質量分析による分子間相互作用解析技術の開発
The nano Pore Optical Interferometry (nPOI) can measure a Kon/Koff histogram on binding kinetics of molecular species in the same manner as SPR, and can identify and quantify compounds/biomolecules even weakly interacting in a complex mixture by mass spectrometry. The nPOI is in principle different from surface plasmon resonance (SPR), and is highly advantageous in its capacity to capture binding molecules 100 times more than the conventional SPR methods, and that nPOI therefore makes it possible to identify weakly interacting molecules which are useful in designing fragment-based and/or medium-size molecules-based drug developments1). In our research group, Carbonic Anhydrase II was immobilized as a ligand protein to the sensor cartridge, six types of low molecular model compounds were applied as analyte, then affinity analysis was performed, and eluted compounds were introduced to nano-ESI LC-MS for verification.