1P-25 PDF
固相誘導体化を用いた低濃度のアミノ酸および有機酸と高濃度の糖類を含む試料の一斉分析法の開発
When the peak of high concentrated sugar is appeared in the chromatogram, it is also difficult to measure both low concentrated amino acids and organic acids. Therefore, by using in-SPE derivatization method technology. At the first sample load, we kept the amino acid and organic acid in SPE cartridge, and the second sample load, less than 1/20 sample amount load into the same SPE cartridge enable to catch the sugar with in the same dynamic range. Thus, we have achieved to analyze for amino acid, organic acid and sugar in simultaneously.