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第1日　5月17日（水）　　P会場（多目的ホール）

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¹³C代謝フラックス解析によるがん細胞中心代謝株間比較

(¹阪大院情報・ ²金沢大がん研)
^o上原ひかる¹・ 荒木千絵¹・ 前田昂亮¹・ 岡橋伸幸¹・ 松田史生¹・ 高橋智聡²・ 清水浩¹

¹³C-metabolic flux analysis (¹³C-MFA) was conducted for a comparison of central carbon metabolism in three human cancer cell lines. MCF7 (human breast cancer) , HeLa (human cervical cancer) and HepG2 (human liver cancer) were cultured in DMEM containing [1-¹³C]glucose. The metabolic flux distributions of three cancer cells were successfully determined from the ¹³C-labeling patterns of intracellular metabolites. The results showed that main source of NADPH for the active cell growth was the reaction catalyzed by acid NADP+ oxidase in all three strains.