We have reported that forest walking results in the modification of autonomic activity and a decreasing blood pressure. Conifer trees produce volatile organic compounds (VOCs) such as isoprenoids and terpenoids (α-, β-pinene, camphene, limonene and bornyl acetate). Anti-inflammatory and anti-cancer effect of essential oils containing geraniol or limonene have been reported1). We have reported the identification of conifer-derived VOCs in the atmosphere of conifer forest and in blood and urine from the conifer forest walkers2). In this presentation, we have tried the determination of α-pinene concentration in the blood or urine from the forest walkers.
Stock solution containing 100mM of α-pinene was prepared in DMSO. Standard α-pinene solution was diluted to 500pM, 5nM, 50nM and 500nM to build the calibration curve. Sample vial was preheating 20 min at 55°C, then headspace adsorption 20min at 55°C by solid phase micro extraction (SPME). The samples were then analyzed by GC/MS.
Results and Conclusion
Linearity of the calibration curve was ranged from 500pM to 500 nM. The concentrations of α-pinene in the blood and urine after forest walking were 3-7nM and 0.1-6nM, respectively.
1) P.L.Crowell. J.Nutr.129,775S-778S(1999)
2) K.Sumitomo, el al., 61th MSSJ Annual Conference, 2P-49 (2013)