2C-O1-1040 PDF
一細胞Orbitrap-MS検出とMSn解裂解析を駆使した植物液胞内二次代謝物の迅速同定法の開発
Flavonoids and their glycosides have large structural diversity due to their complex glycosyl substituents. Among various flavonoid glycosides, we are focusing on isomeric distinguishing on their glycoside regioisomers by means of MSn techniques. We confirmed significant differences in negative MS2 spectra when comparing flavonol 3-O-glycoside and 7-O-glycoside. Furthermore, their MS3 spectra from their aglycones turned out be useful to elucidate their glycosylated positions, which suggested availability for identification of flavonol glycosides. Finally, we attempted our method to live flower cells by using single-cell mass spectrometry technique.