1P-27 PDF
データ非依存的SWATH手法の創薬段階における代謝物探索への応用
Product ion spectra of drug metabolites are important for structure elucidation at the drug discovery stage, and the SWATHTM technique is an information-independent method of obtaining these spectra. A UPLC®-TripleTOF® 5600+ system was used for metabolite profiling of diltiazem as a model compound in cryopreserved hepatocytes. Data obtained via SWATHTM provided useful product ion spectra of metabolites, just as might be obtained using an information-dependent acquisition (IDA) method. SWATHTM was also easier to use and more comprehensive than IDA with regard to measurements of drug metabolites and endogenous compounds.