The 51st Annual Conference on Mass Spectrometry (2003), Tsukuba, Japan

The 51st Annual Conference on Mass Spectrometry (2003), Tsukuba, Japan

  3rd Day Wednesday, May 16, 2003 Program

Auditorium    "Symposium Session"

  Symposium 3 "Young researchers expected in the post-genome era"

  ( 9:30-11:48 ) Chairpersons: Hiroshi Nakayama,Takatoshi Kawai

3-S-01 Mass spectrometric identification of RNA-modifying enzyme genes by ribonucleome analysis
(¹ Grad. Sch. Frontier Sci., Univ. of Tokyo, ² Grad. Sch. Eng., Univ. of Tokyo, ³ Coll. Sci., Rikkyo Univ., ⁴ Grad. Sch. Sci., Tokyo Metropolitan Univ., ⁵ Natl. Inst. Gen., ⁶ Fukuoka Dental Coll., ⁷ Nara Inst. Sci. Tech.)
^oYoshiho Ikeuchi¹, Akiko Noma¹, Akiko Soma³, Naoki Shigi¹, Jun-ichi Kato⁴, Akiko Nishimura⁵, Takeyoshi Miki⁶, Kazuo Kobayashi⁷, Naotaka Ogasawara⁷, Yasuhiko Sekine³, Kimitsuna Watanabe^{1, 2}, Tsutomu Suzuki^{1, 2}

3-S-02 Gel-enhanced LCMS Approaches for Large-scale Proteomics
(Center for Experimental BioInformatics, University of Southern Denmark)
^oYasushi Ishihama, Matthias Mann

3-S-03 Phosphorylation (Post-Translational Modification) Analysis and Structural Analysis by MS
(Univ. Tokushima & RIKEN)
Emiko Yamauchi

3-S-04 Atomic Reconstruction of Metabolism (ARM) Project: Computer Modeling of Metabolic Networks
(AIST, University of Tokyo)
Masanori Arita

3-S-05 Extracting Biomolecular Interaction from Large Biomedical Literature
(HITACHI, Central Research Laboratory)
Yoshihiro Ohta

3-S-06 Cell Simulation: A Fundamental Technology for Systems Biology
(Institute for Advanced Biosciences, Keio University)
Yasuhiro Naito

  Luncheon seminer

  ( 12:00-13:30 ) Applied Biosystems Japan

  Invited lecture 7

  ( 15:00-15:45 ) Chairperson: Toshihide Nishimura

3-T-01 Mass Spectrometry Instrumentation for Proteomics
(Applied Biosystems)
Marvin Vestal

  Special lecture

  ( 15:55-16:40 ) Chairperson: Itsuo Katakuse

3-T-02 The Origin and Future of Soft Laser Desorption
(Shimadzu)
^oKoichi Tanaka

Conference room 1

  Oral presentations "Chiral analysis, Drug, Environmental, Food"

  ( 9:10-9:50 )

3-O1-01 Matrix effects on the IRIS values in FABMS/EL guest method
(¹ Osaka Municipal Techn. Res. Inst., ² ISIR, Osaka Univ., ³ Fac. of Sci., Osaka Univ.)
^oMotohiro Shizuma¹, Hitoshi Yamada², Hiroshi Adachi³, Yoshio Takai², Tokuji Takeda¹, Masami Sawada²

3-O1-02 Mass Spectrometric Chiral Recognition of Heterocyclic Compounds Based on Racemization
(Kansai Univ.)
^oMasayoshi Omoto, Tadashi Shiraiwa, Ryuichi Arakawa

  ( 9:50-10:30 )

3-O1-03 Optimization of automated pretreatment conditions for analysis of drugs in plasma by liquid chromatographymass spectrometry
(¹ Shimadzu Corporation, ² Eisai Co., Ltd.)
^oShin-ichi Kawano¹,Hiroyuki Murakita¹, Eiichi Yamamoto², Naoki Asakawa²

3-O1-04 Strategy for structure elucidation of drug metabolites derived from protonated molecules and (MS)_n fragmentation by ESI Ion Trap MS
(Fujisawa Pharmaceutical Co., Ltd.)
^oZenzaburo Tozuka, Hayato Kaneko, Toshifumi Shiraga, Akio Kawamura, Akira Kagayama

  ( 10:30-11:10 )

3-O1-05 Analysis of 3,3-dichlorobenzidine by LC/MS/MS
(Fac. of Engineering, Kansai Univ.)
Yasuo Mizooku, Hiroyuki Hashimoto, Ryuichi Arakawa

3-O1-06 Detection of Chlordane by LC/MS
(¹ Osaka City Institute for Public Health & Environmental Sciences, ² Kansai Univ.)
^oHiroshi Moriwaki¹, Hiroyuki Hashimoto², Ryuichi Arakawa²

  ( 11:10-11:50 )

3-O1-07 Application of on-line electrospray ionization mass spectrometry with a micro-flow electrolytic cell: Dimerization of caffeic acid
(¹ Fac. of Eng., Kansai Univ., ² Fac. of Science, Kobe Univ., ³ Kimoto Electric Co.)
^oMasashi Yamaguchi¹, Hiroki Hotta², Toshiyuki Osakai², Takeshi Kimoto³, Ryuuichi Arakawa¹

3-O1-08 Interpretation of Imojoutyu (Japanese sweet potato spirit) flavor using mass spectrum analysis
(¹ Dept. of Eng., Towa Univ., ² Dept. of Horticulture, Chiba Univ.)
^oTakehiko Tsuruta¹, (late) Takeo Ohta²

  Luncheon seminer

  ( 12:00-13:30 ) GL Sciences

Foyer & Poster room

  Poster presentations

  ( 13:30-15:00 ) Chairperson: Toshihide Nishimura

3-P-01 Use of mass spectral data base of SDBS by the internet system
(National Institute of Advanced Industrial Science and Technology)
^oNobuhide Wasada, Shinichi Kinugasa, Takeshi Saito, Yuuko Takizawa, Mari Kusakabe, Keiichiro Ishikawa

3-P-02 Information Fining in Biomedical Literature
(Hitachi, Ltd., Central Research Laboratory)
^oYoshihiro Ohta

3-P-03 Cell Simulation: A Fundamental Technology for Systems Biology
(Institute for Advanced Biosciences, Keio Univ.)
Yasuhiro Naito

3-P-04 Bioinformatics of Focused Proteome1: Performance Comparison of Protein Identification Tools for LC-MS/MS Data
(¹ Mitsui Knowledge Industry Co., Ltd., ² Eisai Co., Ltd.)
^oYasuto Yokoi¹, Yuji Miura¹, Ken Aoshima¹, Tsuyoshi Tabata², Takatoshi Kawai², Takeshi Nagasu², Hiroyuki Katayama², Yoshiya Oda²

3-P-05 Bioinformatics of Focused Proteome2: Filtering Tools for Protein Identification Results
(¹ Eisai Co., Ltd., ² Mitsui Knowledge Industry Co., Ltd.)
^oTsuyoshi Tabata¹, Takatoshi Kawai¹, Takeshi Nagasu¹, Hiroyuki Katayama¹, Yoshiya Oda¹, Yasuto Yokoi², Yuji Miura², Ken Aoshima²

3-P-06 Proteomic analysis of human proteins using consensus EST database
(¹ Graduate School of Frontier Sciences, Univ. of Tokyo, ² Maze Inc., ³ Sakaguchi Giken Inc., ⁴ National Institute of Radiological Sciences)
^oYuriko Sakaguchi¹, Yasuji Kasama², Kazuhiro Kondo², Junko Ozaki², Masaaki Sakaguchi³, Toshiyuki Saito⁴, Tsutomu Suzuki¹

3-P-07 Electrophoretic profiles of phosphorylated protein in rat hepatic stellate cells
(Biomolecular Characterization Division, RIKEN)
^oHiromi Miura, Koji Takio, Hiroshi Nakayama

3-P-08 Proteome analysis of Escherichia coli by SDSPAGE and 2-Dimensional LC/MS/MS
(¹ Japan Science and Technology Corporation, ² MDS Proteomics, ³ TOWA KAGAKU Co., Ltd., ⁴ Prophoenix Co., Ltd., ⁵ Third Department of Internal Medicine, Osaka City University Medical School, ⁶ Cooperative Link of Unique Science and Technology for Economy Revitalization (CLUSTER) Hiroshima Prefectural Institute of Industrial Science and Technology, ⁷ Developmental Biology Laboratory, Department of Biological Science, Graduate School of Science, Hiroshima University)
^oYuka Myoken-Miyamoto¹, Dan Bach Kristensen², Yoshiko Takeda¹, Akira Yamagata^{1, 3, 4} , Norifumi Kawada⁵, Chise Tateno⁶, Ken Oofusa^{1, 3, 4}, Katsutoshi Yoshizato^{1, 6, 7}

3-P-09 Screening for the phosphorylated peptides with Q/Linear Ion Trap hybrid mass spectrometer
(Takara Bio Inc.)
^oYoshifumi Kogure, Jun Watanabe, Yasutoshi Kawase

3-P-10 A novel phosphate capture molecule "Phos-tag^TM" for mass analysis of phosphoproteins
(¹ Nard Institute, Ltd., ² MANAC Incorporated., ³ Hiroshima Univ.)
^oNorio Minami¹, Akihiko Kawasaki¹, Hironori Takeda², Tohru Koike³

3-P-11 Quantitative proteomics of phospho-peptides by modification with stable isotope derivatives
(¹ Faculty of Pharmaceutical Sciences, Nagoya City Univ., ² Faculty of Medicine, The Univ. of Tokyo)
^oChie Murata^{1, 2}, Kohfuku Kohda¹, Masayoshi Imagawa¹, Ryo Taguchi^{1, 2}

3-P-12 Myosin Phosphorylation by Death-Associated Protein Kinase
(¹ Nagasaki Univ., Schl. Env., ² Mitsubishi Pharma Co., ³ Osaka Univ., Protein Inst.)
^oTakayuki Miyanishi¹, Eisuke Sawada¹, Takeshi Hioki², Takehisa Ishii², Nobuaki Okumura³, Toshifumi Takao³

3-P-13 Mass Spectrometry of S-Nitosoprotein. II. Requirement of fine settings of ESI conditions
(Osaka Medical Center and Research Institute for Maternal and Child Health)
^oRina Kaneko, Yoshinao Wada

3-P-14 Implication of S-nitrosylation in the process of mixed disulfide formation
(Osaka Medical Center and Research Institute for Maternal and Child Health)
^oYoshinao Wada, Rina Kaneko

3-P-15 Analysis of endocrine disruptive action by nonylphenol and bisphenol A
(Kobe Pharmaceutical Univ.)
^oAtsuko Takeuchi, Kayoko Saiki

3-P-16 Screening of peptide sequences bound bio-active compound using phage display technology
Tokyo University of Science)
^oSatoko Aoki, Keisuke Ohta, Katsunori Suzuki, Takayuki Yamazaki, Fumio Sugawara, Kengo Sakaguchi

3-P-17 A Methodology for Rapid and Efficient Characterization of Novel Ghrelin-derived Peptides by Mass Spectrometry: MALDI-TOF-MS, MALDI-TOF-TOF-MS and Ladder Sequencing
(¹ Daiichi Suntory Pharma Co., Ltd., ² National Cardiovascular Center Research Institute, ³ Suntory Institute for Bioorganic Research)
^oKeiko Iwasa¹, Osamu Tsunoka¹, Ryoji Muramatsu¹, Yujiro Hayashi¹, Yoshiharu Minamitake¹, Kenji Kangawa², Hideo Naoki³, Terumi Nakajima³

3-P-18 Multifunctional Sample Pretreatment Using Stop and Go Extraction Tips (StageTips) for Proteomics
(Center for Experimental BioInformatics, Univ. of Southern Denmark)
^oYasushi Ishihama, Juri Rappsilber, Matthias Mann

3-P-19 Drug target identification by chemical-targeted proteome
(Laboratory of Seeds Finding Technology, Eisai Co., Ltd.)
^oToshitaka Sato, Yoshiya Oda, Takashi Owa, Hiroyuki Katayama, Akira Yokoi, Jyunro Kuromitsu, Takeshi Nagasu

3-P-20 Chemical targeted proteome: Identification and characterization of phosphodiesterase V from platelets lysate
(Lab. of Seeds Finding Technology)
Kishan Agarwala, Yoshiya Oda, Yasutaka Takase, Hiroyuki Kato, Toshitaka Sato, Takeshi Nagasu

3-P-21 UV-MALDI-TOF-MS analysis of the oligosaccharides from the cysteine proteinase cruzipain
(¹ College of Agriculture, Ehime University, Japan, ² Instituto de Investigaciones Biotecnologicas, Universidad Nacional de Gral., San Martin, Argentina, ³ Cihidecar-Conicet, Departamento de Quimica Organica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina)
^oYuko Fukuyama¹, Mariana Barboza², Vilma Duschak², Hiroshi Nonami¹, Rosa Erra-Balsells³, Juan J. Cazzulo², Alicia S. Couto³

3-P-22 Linkage analysis of oligosaccharides by using negative-mode MALDI-MS
(Department of Chemistry, School of Science, The University of Tokyo)
^oTohru Yamagaki

3-P-23 Distinguishing between isomeric oligosaccharides by mass spectrometry
(¹ Department of Chemistry, School of Science, The University of Tokyo, ² National Institute of Advanced Industrial Science and Technology)
^oTohru Yamagaki¹, Hiroshi Nakanishi²

3-P-24 LC/MS applied to ganglioside
(IARC, Tokyo Medical and Dental University)
^oTakeshi Kasama, Kenji Kawasaki

3-P-25 Photochemistry, ESI-MS, Absorption and Luminescence Spectroscopy as Complementary Tools in UV-MALDI-MS Analysis of Carbohydrates
(¹ College of Agriculture, Ehime Univ., Japan, ² Cihidecar-Conicet, Departamento de Quimica Organica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina)
^oYoko Yasuda¹, Olga I. Tarzi², Hiroshi Nonami², Rosa Erra-Balsells²

3-P-26 UV-MALDI-TOF-MSAnalysisofN-Sub-stituted Monosaccharides by Using Different Matrices
(¹ College of Agriculture, Ehime Univ., Japan, ² Cihidecar-Conicet, Departamento de Quimica Organica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina)
Yasuto Sato¹, Maria C. Matulewicz², Carlos A. Stortz², Alberto S. Cerezo², Hiroshi Nonami¹, Rosa Erra-Balsells²

3-P-27 Cell map metabolomics on molecular species of phospholipids
(¹ Graduate School of Pharmaceutical Sciences, Nagoya City Univ., ² Graduate School of Medicine, The Univ. of Tokyo, ³ Graduate School of Medicine, Nagoya Univ.)
^oToshiaki Houjou^{1, 2}, Toyoshi Fujimoto³, Ryo Taguchi²

3-P-28 Qantitative analysis of Prostaglandin J₂ family made by culture cell with LC-ESI-QMS
(¹ Osaka Bioscience Institute, ² Ehime Univ. School of Medicine)
^oToshihiko Maruyama¹, Kosuke Aritake¹, Yoshihiro Urade¹, Maiko Emi², Kazutaka Maeyama²

3-P-29 Analysis of Prostaglandins by LC/MS
(Department of Biochemistry and Molecular Biology, Faculty of Medicine, Graduate School of Medicine, Univ. of Tokyo)
^oToshie Takahashi, Yoshihiro Kita, Naonori Uozumi, Takao Shimizu

3-P-30 Comparison of mass spectrum of sphingophosphonolipids with various ionizations
(¹ Kansai Lipid Institute, ² Joint Research Center, Kinki Univ.)
^oHideki Kishine¹, Masanori Morita², Akira Hayashi¹

3-P-31 Normalphasecapillarymonolithiccol-umn for lipid analysis
(IARC, Tokyo Medical and Dental Univ.)
^oKenji Kawasaki, Takeshi Kasama

3-P-32 Identification of Bile Acids as Endogeneous Ligands of Novel oGPCR, BG37
(¹ Applied Biosystems Japan Ltd., ² Nagoya City Univ., ³ The Univ. of Tokyo)
^oKaoru Karasawa¹, Mayuko Ishida^{2, 3}, Toshiaki Houjou^{2, 3}, Ryo Taguchi³

3-P-33 Rapid profiling and characterization of phospholipids using new liner ion trap MS technology
(¹ Applied Biosystems Japan Ltd., ² Nagoya City Univ., ³ The Univ. of Tokyo)
^oKaoru Karasawa¹, Mayuko Ishida^{2, 3}, Toshiaki Houjou^{2, 3}, Ryo Taguchi^3q/sup

3-P-34 Formation and destruction of the guanine quartet in solution observed by CSI-MS
(¹ Chemical Analysis Center, Chiba Univ., ² Department of Synthetic Chemistry and Biochemistry, and Faculty of Engineering)
Shigeru Sakamoto¹, Kazuhiko Nakatani², Isao Saito², ^oKentaro Yamaguchi¹

3-P-35 Comparative UV-MALDI-TOF-MS Analysis of New Synthetic Nucleosides Using Different Matrices
(¹ College of Agriculture, Ehime University, Japan, ² Cihidecar-Conicet, Departamento de Quimica Organica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina, ³ Centro de Estudios e Investigaciones, Universidad Nacional de Quil-mes, Argentina)
Iku Ninomiya¹, Maria A. Ponce², Luis E. Iglesias³, Adolfo M. Iribarren⁴, Hiroshi Nonami a and Rosa Erra-Balsells²

3-P-36 Extraction and LC-MS analysis of flavonoids in onions
(¹ National Food Research Institute, ² Hokkaido Agricultural Center)
^oKimiko Yabe¹, Sachiko Hoshino¹, Hiroyuki Nakagawa¹, Takako Najiwara¹, Shioka Hamamatsu¹, Yuji Noguchi²

3-P-37 Identification of chemotypes among Perilla species by GC/MS
(¹ National Food Research Institute, ² National Institute of Agrobiological Sciences)
^oHidetaka Kobayashi¹, Miyuki Nitta², Mayumi Kameyama-Ohnishi¹, Tsukasa Nagamine², Mitsuru Yoshida¹

3-P-38 Analysis of low boiling volatile compounds in sweet potato by SPME-GC-MS
(¹ Faculty of Engineering, Kansai Univ., ² Nippon Food Development Co.)
^oMuneyoshi Yoshikawa¹, Yumiko Miyamoto¹, Yasuo Mizooku¹, Toshiyuki Ozaki², Ryuichi Arakawa¹

3-P-39 The usages sodium adduct ions with LC/ESI-MS for the quantitative analysis of type A- and B-trichothecens
(¹ Nihon Waters K. K., ² Graduate School of Natural Science and Engineering, Kanazawa Univ.)
^oHideki Sasaki¹, Jun Yonekubo¹, Kazuichi Hayakawa²

3-P-40 Investigation of analytical methods for acrylamide in foods by LC-MS/MS
(JASCO Interantional Co., Ltd.)
^oRyo Ogasawara, Fusako Kaneda, Hideto Kakimi

3-P-41 Diagnostics of plasma for low-k material deposition by monomer-vapor polymerization technique (1)
(¹ MIRAI projectASET, ² AIST, ³ MIRAI projectASRC, AIST, ⁴ RCNS, Hiroshima Univ.)
^oKeizo Kinoshita¹, Akinori Nakano¹, Jun Kawahara¹, Naoaki Saito², Takamaro Kikkawa^{3, 4}

3-P-42 3D Spectra of Uniform Synthetic Polymers by High-Resolution Ion-Mobility TOF-MS. Effects of Stereoregularity and Adduct Ions
(¹ Fac. of Engineering, Kansai Univ., ² Graduate School of Engineering Science, Osaka Univ., ³ Dept. of Chem., Indiana Univ.)
^oRyuichi Arakawa¹, Masanobu Kondou¹, Kouichi Ute², A. Hilderbrand³, D. E. Clemmer³

3-P-43 HCl Loss from the Molecular Ions of Chlorophenols Using Charge Inversion Mass Spectrometry. Comparison with MIKE Spectrometry
(¹ JEOL Ltd., ² Osaka Prefecture Univ.)
^oYutaka Takahashi¹, Nobutake Kabuki², Yoshiaki Kawamura², Akihiro Kitaguchi², Shigeo Hayakawa²

3-P-44 Dissociative reaction of polychlorinated phenol anions
(Tokyo Metropolitan Industrial Technology Research Institute)
Seiko Nakagawa

3-P-45 Dissociation of neutral metal carbonyls by using charge inversion mass spectrometry
(College of Integrated Arts & Sciences, Osaka Prefecture Univ.)
Keisuke Tominaga, Shinnya Konishi, ^oShigeo Hayakawa

3-P-46 Identification of the Characteristic Fragmentation Processes of Alkali Halide Triple Ion Adduct Monosaccharide Ions by CAD
(¹ Graduate School of Engineering, Univ. of Fukui, ² Faculty of Education and Regional Studies, Univ. of Fukui)
^oNora Martinez¹, Kimio Isa², Ryuuji Nakata², Minoru Hatanaka¹

3-P-47 Structural Analysis of Protonated Dipeptide Ions by High Performance CID Spectra (2)
(¹ Facluty of Education and Regional Studies, Univ. of Fukui, ² Nicca Chemical Co., Ltd.)
^oKimio Isa¹, Makiko Gotou¹, Hideyuki Takezawa¹, Noriaki Kobayashi²

3-P-48 Pulsed-gas-introduction infrared multiphoton dissociation in a quadrupole ion trap
(Hitachi, Ltd., Central Research Laboratory)
^oHideki Hasegawa, Yuichiro Hashimoto, Izumi Waki

3-P-49 Gallium Ion Attachment Mass Spectrometry (Ga^{-IAMS) of Organic Molecules
(¹ Oyama National College of Technology, ² Anelva Corporation)
^oHiroshi Kishi¹, Toshihiro Fujii²

3-P-50 Comparison between excitation waveforms in an FT-ICR mass spectrometer by many-particle simulation
(Dept. of Computer Eng., Hiroshima City Univ.)
Makoto Fujiwara

3-P-51 Vapor pressure measurement of LiNbO₃ with High Temperature Mass Spectrometer
(¹ Research Center for Nuclear Science and Technology, The Univ. of Tokyo, ² Graduate School of Engineering, The Univ. of Tokyo)
^oMasaru Yasumoto¹, Tsuyoshi Hoshino², Michio Yamawaki², Takayuki Terai²

3-P-52 Degradation Pathways of Acetylcholine Cluster Ions
(Graduate School of Integrated Science, Yokohama City Univ.)
^oNaoto Soya, Mitsuo Takayama

3-P-53 Measurement of molecular weight of a cholic acid micelle
(Faculty of Engineering, Gifu Univ.)
^oTakatomo Kajiura, Daisuke Nohara