Poster Presentations
Day 3, June 12（Fri.） Room P (5F 501+502)
- 3P-50
Development and Multicomponent Extension of a Nano-LC/High-Resolution MS Quantification Method for Laser Microdissection Micro-Sample
(1RIKEN CSRS, 2Tokyo Univ. Technol., 3Univ. Ryukyus, TBRC)
oYumiko Takebayashi1, Hiromi Suzuki1,2, Masami Hirai1, Mitsunori Seo1,3
Quantitative analysis of plant hormones at cellular and tissue levels remains challenging due to their extremely low endogenous concentrations. Although LC/MS analysis of small samples prepared by laser microdissection (LMD) has been reported, such approaches often require a large number of tissue sections, resulting in low throughput.
In this study, we developed a highly sensitive quantification method for small samples by combining nano-flow liquid chromatography (Nano-LC) with high-resolution mass spectrometry. This approach significantly improved the detection limit for quantification of the major auxin indole-3-acetic acid (IAA) and markedly reduced the number of LMD sections required for reliable quantification.
The established platform enabled sensitive and reproducible quantification of IAA in small regions while maintaining spatial resolution. Furthermore, tracer experiments using stable isotope-labeled tryptophan applied to maize coleoptiles successfully enabled the detection and quantification of labeled IAA derived from the Trp produced in situ, validating the effectiveness of the method. Currently, this platform has been applied to the quantification of other plant hormones and related metabolites. This method provides a robust mass spectrometry–based strategy for plant hormone analysis at high spatial resolution.