Poster Presentations
Day 3, June 12（Fri.） Room P (5F 501+502)
- 3P-41
Strategies for Rapid Intact Mass Screening Analysis: Achieving One-Second per Well
(1Genedata AG, 2AB SCIEX LLC, 3Beckman Coulter Life Sciences, 4Genedata Ltd.)
oNatalie Weijia Zhong1, Juergen Kastler1, Rahul Baghla2, Sarah Simons3, Jon Costello4, Matthew Green4
With emerging high-speed mass spectrometry (HT-MS) platforms the sample-to-report bottlenecks have shifted beyond acquisition. This is especially true for intact mass analysis, which requires deconvolution, peak detection, quantification, and hit calling steps. This study explores the major factors that affect analysis speed across various protein sizes on a fully integrated and automated SCIEX Echo® MS+ system. Maximum analysis speed depends on assay requirements. In large screening assays, lower mass accuracy demands can trade off for faster analysis. Protein size also influences whether isotopic resolution is obtained, impacting analysis strategy and speed. We profiled pre-processing, deconvolution, and hardware to identify key considerations for optimizing analysis speed of the end-to-end process.
Process mapping on protein standards shows the factors contributing to speed. Server-based installations, enterprise integrations, triggering automation, and suitable software achieve speeds comparable to acquisition. Hardware and metadata integrations are often overlooked as significant factors. Deconvolution is frequently seen as a bottleneck due to software not optimized for high-throughput. Frequently, a semi-automated approach is required. At scale, this requires user interfaces designed for big data and tools to help the user interpret analysis quickly. Faster speeds unlock large-scale assay strategies and additionally benefit data integrity, reproducibility, and downstream cross-dataset integration & reusability.