Poster Presentations
Day 3, June 12（Fri.） Room P (5F 501+502)
- 3P-33
Comparative N-Glycomics of 2D- and 3D-Cultured Colorectal Cancer Cell Lines
(1TMIG, 2Shimadzu)
oHiroki Tsumoto1, Takashi Nishikaze2, Yuuki Shichi1, Keitaro Umezawa1, Kyojiro Kawakami1, Toshiyuki Ishiwata1, Yoshihiro Hayakawa2, Yuri Miura1
Colorectal cancer (CRC) exhibits epithelial–mesenchymal heterogeneity linked to malignancy. We performed N-glycomics of five CRC cell lines under 2D and 3D cultures using sialic acid linkage-specific alkyl amidation (SALSA). Abundances of complex-type were remarkably increased in 3D-cultured in comparison with 2D-cultured. In contrast, high-mannose type in 3D-cultured were not changed. It was suggested that complex-type N-glycans play an important role in the formation of CRC spheres.