Poster Presentations
Day 3, June 12（Fri.） Room P (5F 501+502)
- 3P-31
Lectin-IMC: A multiplexed imaging mass cytometry approach for spatial validation of glycan signatures and carrier glycoproteins
(AIST)
oChiaki Nagai-okatani, Umeko Horiuchi, Patcharaporn Boottanun, Kei Miyako, Atsushi Kuno
Protein glycosylation regulates protein function and cell–cell interactions, and disease-associated alterations in glycan structures have emerged as promising targets for glycan-based drug discovery. Understanding their biological and pathological significance requires not only structural characterization of glycans but also identification of their carrier glycoproteins and their spatial localization within tissues. This has led to the concept of spatial glycomics/glycoproteomics, which integrates glycan, protein, and spatial information to elucidate disease-associated glycosylation in situ.
Here, we present Lectin-IMC, a lectin-assisted imaging approach based on imaging mass cytometry, as a key technology for achieving spatial glycomics/glycoproteomics. Lectin-IMC enables simultaneous visualization of glycans, carrier glycoproteins, and cell-type markers at micrometer-scale resolution within tissues. Integrated with lectin microarray–based glycan profiling and MS-based glycoproteomics, Lectin-IMC provides the spatial layer needed to validate glycan signatures identified by in-solution analyses. Using this framework, we examined fibrotic regions in a dilated cardiomyopathy (DCM) mouse model, enabling spatial validation of disease-associated glycan alterations and their carrier glycoproteins.
This multimodal glycoprotein analysis strategy links glycan changes to their carriers and spatial context, providing insight into disease-related glycosylation and supporting biomarker discovery and glycan-based therapeutic development.