Poster Presentations
Day 3, June 12（Fri.） Room P (5F 501+502)
- 3P-24
Development of a Simultaneous Multi-Residue Analytical Method for Antiprotozoal drugs in Livestock using LC-MS/MS
(MFDS Korea)
oHyojin Chang, Yeong-ju Jo, Ye-Bin Jang
Antiprotozoal drugs are extensively used in livestock to prevent and control protozoal infection and to improve animal productivity. However, the misuse or overuse of these veterinary drugs may result in residue contamination in animal-derived foods, raising concerns regarding food safety and potential risks to human health. Therefore, reliable and sensitive analytical methods are required for effective monitoring of antiprotozoal drug residues in livestock.
In this study, a simultaneous multi-residue analytical method was developed for the determination of seven antiprotozoal drugs, including clopidol, in aniaml-derived foods, including beef, pork, chicken, milk and fat, using liquid chromatography-tandem mass spectrometry(LC-MS/MS). Samples were extracted and purified using an optimized sample preparation procedure and analyzed under multiple reaction monitoring(MRM) mode.
Each compound was validated at three levels, 0.5×MRL, 1×MRL, 2×MRL(maximum residual limit) or 1×LOQ, 2×LOQ, 10×LOQ(limit of quantification) according to the CODEX guidelines(CAC/GL 71-2009). The method demonstrated a correlation coefficient greater than 0.99, with an average recovery and a coefficient of variation that met the requirements of the CODEX guidelines. This method is expected to improve the analytical efficiency of antiprotozoal drug residue detection in livestock, thereby strengthening the safety management.