Poster Presentations
Day 3, June 12（Fri.） Room P (5F 501+502)
- 3P-23
Examination of Analytical Conditions for Separation of Long-chain Synthetic Oligonucleotides using LC/MS
(Tosoh)
oSeiji Ito
The ion-pair reversed phase chromatography (IP-RPC) with eluents containing alkylamine and hexafluoroisopropanol (HFIP) has been widely used for LC/MS analysis of synthetic oligonucleotides. However, under such conditions, there were concerns that the ion source might be contaminated by ion-pairing reagents, or the selectivity of homologues or isomers was lower than that of ion exchange chromatography (IEC). Previous studies have investigated the use of trofluoroethanol (TFE) as an alternative solvent to HFIP and the modification of the separation mode, but because the analytes used were nucleic acids approximately 20 mer in length, there was insufficient knowledge about the separation of long-chain oligonucleotides, for which further development is expected in the future.
In this study, the analytical conditions for long-chain oligonucleotides were optimized by using TFE as additives in eluent. Furthermore, the effects of analytical conditions on IEC separation were also evaluated, with the aim of applying IEC to 2D-LC analysis by combining IEC and RPC.