Poster Presentations
Day 3, June 12（Fri.） Room P (5F 501+502)
- 3P-22
Rapid and reliable structural confirmation and comprehensive impurity analysis of cyclic peptides
(SCIEX)
oKaoru Karasawa
Development of cyclic peptides is rapidly gaining not only for new therapeutic modality but DDS systems. The sequence confirmation and in-depth impurity analysis are important due to their high specificity. But the analysis is complex because of the molecular size, the cyclic structures, composed isomeric amino acids and various modifications, and lack of processing software.
This poster reports on the rapid structural confirmation and comprehensive analysis of CysA-related impurities of a representative cyclic peptide drug, cyclosporine A (CysA), using the ZenoTOF 7600 system integrated with Molecule Profiler software and SCIEX OS software. MS-level identification and MS/MS-level structural confirmation, including unexpected impurities, were demonstrated at concentrations as low as 0.01% (w/w) and 0.04% (w/w), respectively. Based on MS and MS/MS fragmentation, impurities assigned as isocyclosporine A (IsoCysA) and cyclosporine C (CysC) were determined by co-elution and MS/MS comparison with authentic standards. The unexpected impurity detected as a CysA-related impurity, which generated at least five common product ions with CysA, was presumed to be butenylmethylthreonine (Bmt) with loss of C3H4O.