Poster Presentations
Day 3, June 12（Fri.） Room P (5F 501+502)
- 3P-08
Evaluation of Potential Risks of Food Components Using Antioxidant Enzyme-treated Cellular Systems Integrated with Metabolic Profiling
(Kyushu Univ.)
oTomomi Ichinose, Seong-uk Lee, Wataru Isogai, Motofumi Kumazoe, Hirofumi Tachibana, Yoshinori Fujimura
Non-physiologically high concentrations of epigallocatechin-3-O-gallate (EGCG), a major green tea catechin, can induce hepatotoxicity. However, the actual cellular effects of EGCG itself remain unclear, because reactive oxygen species (ROS) are generated through auto-oxidation in cellular system. In this study, hepatocellular growth and viability in response to EGCG were evaluated under ROS-free conditions created by antioxidant enzymes (superoxide dismutase (SOD) and catalase (CAT)) in normal murine liver cell line NMuLi. Although EGCG at high concentration (> 20 μM) suppressed ATP level as indicators of cell growth and viability, this effect was abolished by SOD/CAT treatment. Under ROS-free conditions, physiologically relevant EGCG (< 5 μM) didn’t affect ATP level. Using a chemometric approach, metabolic profiling (MP) analysis of multiple green tea extracts allowed screening of six compounds that synergistically reduced ATP levels in combination with EGCG (5 μM). The synergistic effects of EGCG and six compounds observed in NMuLi cells could also be detected in HepaSH cells, which were cultured in an environment mimicking the human hepatocyte proliferative niche within chimeric mice. These findings suggest effectiveness of employing MP method with SOD/CAT-treated cellular systems to find compound combinations associated with the potential hepatotoxicity of EGCG.