Fundamental Sessions
Day 3, June 12（Fri.） 9:51-10:09 Room D (5F 511+512)
- 3D-O1-0951（1P-09）
Mass Spectrometry Imaging of Cardiac Tissue in a Dilated Cardiomyopathy Mouse Model using t-SPESI with Chemically Modified Probe
(1UOsaka, 2UOsaka, 3UOsaka)
oTakao Yasuda1, Tasuku Kato1, Yoichi Otsuka1, Shuichi Shimma2, Hidetaka Kioka3, Yasushi Sakata3, Michisato Toyoda1
To enable long-term stable mass spectrometry imaging (MSI) by t-SPESI, the probe surface was chemically modified with a fluorinated compound to suppress the adsorption of biological components onto the quartz capillary probe. Consequently, temporal fluctuations in the ion signal intensity and the accumulation of biomolecules at the probe tip were effectively suppressed. The effectiveness of the modified probe was evaluated using MSI of cardiac tissue from a dilated cardiomyopathy mouse model. Lipid species localized in fibrotic and non-fibrotic regions were successfully visualized. Notably, reduced ion signal intensity of acylcarnitine were observed in regions surrounding the fibrotic areas.