Symposium Sessions
Day 3, June 12（Fri.） 10:15-10:45 Room B (4F 411+412)
- 3B-S1-1015
Proteomics of hematopoietic stem cells towards reversing immunosenescence
(DGIST)
oMin-Sik Kim
Hematopoietic stem cells (HSCs) orchestrate lifelong blood cell production through the process of hematopoiesis. As HSCs age, they undergo functional deterioration such as reduced self-renewal, delayed activation, and lineage output imbalance. Recent studies suggest that aging-associated dysfunctions may be reversible, highlighting the need to investigate underlying molecular mechanisms during HSC aging. Despite several studies, the proteomic landscape underpinning these changes remains insufficiently explored. To address this gap, we performed high-resolution mass spectrometry-based quantitative proteomic profiling of HSCs isolated from young (2-month-old) and aged (18-month-old) mice. Comparative analysis identified 492 proteins significantly altered with age, of which were found to be associated with oxidative phosphorylation, and DNA repair pathways revealed by bioinformatics analyses. In vivo functional validation confirmed that the inhibition of one of the processes or signaling pathways significantly attenuates aging-associated deficits in hematopoietic stem cells. In conclusion, our study defines the age-related proteomic remodeling of HSCs and identifies a key regulator to restore the HSC function.