Symposium Sessions
Day 3, June 12（Fri.） 9:15-9:45 Room B (4F 411+412)
- 3B-S1-0915
Microfluidic Single-Cell Proteomics and Multi-Omics Analysis
(ZJU)
oQun Fang
Performing proteomics analysis at the single-cell level presents significant technical challenges.
In 2018, by integrating the microfluidic sequential operation droplet array (SODA) technique previously developed by our group with mass spectrometry-based shotgun proteomics technique, for the first time we achieved single-cell proteomics analysis of mammalian somatic cells. Based on the SODA technique, we further developed an automated single-cell sorting, capture, and pretreatment instrument. By integrating this instrument with LC-MS/MS, we developed a pick-up single-cell proteome analysis (PiSPA) workflow for quantifying up to 3000 proteins in a mammalian cell.
We have successfully applied these systems to deep-coverage single-cell proteomics analysis, integrated single-cell transcriptomics-proteomics analysis, combined single-cell proteomics-metabolomics analysis, as well as immune cell-tumor cell interaction analysis, tumor cell drug resistance analysis, and circulating tumor cell (CTC) analysis in clinical samples at the single cell level. Recently, we also established a benchmark informatics workflow for single-cell proteomics analysis. Based on these techniques and systems, we developed the commercial microfluidic single-cell manipulation instrument—the Celleagle system, and identified over 7,000 proteins from a single A549 cell, achieving single-cell proteomics analysis with even higher depth coverage.