Poster Presentations
Day 2, June 11（Thu.） Room P (5F 501+502)
- 2P-42
Temperature-controlled native MS for evaluation of structural stability of proteins
(AIST)
oKazumi Saikusa, Tomoya Kinumi
Native mass spectrometry (native MS) is an analytical tool for determining the masses of proteins and protein complexes while preserving interactions in solution without cross-linking. Traditionally, native MS has provided only mass information; however, temperature-controlled native MS has been developed to analyze the structural stability of biomolecules in solution. In this study, we constructed a temperature-controlled nanoelectrospray ionization (nanoESI) source and analyzed the model protein ubiquitin using it. As a result, the temperature-controlled nanoESI source was successfully constructed. In addition, the presentation will introduce applications of temperature-controlled native MS to monoclonal antibodies.