Program

Poster Presentations

Day 2, June 11（Thu.）　　Room P (5F 501+502)

2P-24

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Rapid Analysis of Yeast Culture Supernatants Using PESI-MS

(¹UOsaka, ²UOsaka, ³Kobe Univ., ⁴Shimadzu, ⁵Kobe Univ.)
^oTakeo Taniguchi¹, Nobuyuki Okahashi^1,2, Prihardi Kahar³, Takanari Hatori⁴, Chiaki Ogino⁵, Hidenori Takahashi⁴, Fumio Matsuda^1,2

Probe electrospray ionization-mass spectrometry (PESI-MS) directly ionizes metabolites adhering to the tip of a probe by applying a high voltage. The method is expected to enable rapid analysis of samples containing large amounts of salts, such as culture supernatants, since the injection of picolitre-level sample volumes minimizes the risk of mass spectrometer contamination. Here, we evaluated a high-throughput method for the analysis of spent medium supernatant from yeasts culture using PESI-MS. Measurement stability and analysis time of amino acids, organic acids and sugar alcohols were investigated. The levels of metabolites in 126 yeast culture supernatants (14 yeast strains × 3 culture conditions × biological triplicates) were measured at 15 seconds per sample, demonstrating the utility of PESI-MS for the analysis of samples containing salts.

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