Program

Poster Presentations

Day 2, June 11（Thu.）　　Room P (5F 501+502)

2P-23

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Development of a Method for Single-cell Amino Acid Analysis Using Derivatization.

(¹Meijo Univ., ²Univ. Shizuoka, ³Ajinomoto)
^oSeiya Yonezu¹, Aogu Furusho², Akiho Murai³, Kotoe Nakasha³, Akihiro Arakawa³, Sachise Karakawa³, Eiji Sugiyama¹, Kenichiro Todoroki², Hajime Mizuno¹

Amino acids play an important role in a wide range of cellular functions, and analyzing amino acids in cells is effective for detailed analysis of various cellular states and the elucidation of pathological conditions. Conventional metabolomics methods using a large number of cells provide average information on cell populations, and it is not possible to obtain information on individual cells. Amino acid analysis at the single-cell level is necessary because it is difficult to detect trace amounts of amino acids by conventional methods. In this study, we aimed to enable amino acid detection at the single-cell level by improving ionization efficiency by amino acid derivatization, evaluating the sample preparation method, and optimizing　LC-MS measurement conditions. The derivatization reagent useds is p-N,N,N-trimethylammonioanilyl N′-hydroxysuccinimidyl carbamate iodide (TAHS). This reacts with the amino group of amino acids to add imparts a positive charge, thereby improving the ionization efficiency during mass spectrometry.

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