Program

Poster Presentations

Day 2, June 11（Thu.）　　Room P (5F 501+502)

2P-22

PDF

Development of Analytical Method of Carbon Stable Isotope Ratios in Amino Acid/Nucleotide Using LC-IRMS

(Shibaura Inst. Tech.)
^oJihoon Kim, Hiroto Kawashima

Amino acids and nucleotides are the fundamental components of proteins and nucleic acids, serving as major carbon sources and key intermediates in metabolic pathways. The carbon stable isotope ratio (δ¹³C) is widely used as an important tool in geochemistry and ecology. Compound-specific δ¹³C analysis using Liquid chromatography isotope ratio mass spectrometer (LC-IRMS) was established in 2004 and has been applied to various field e.g., food science and archaeological science. This study aimed to develop a compound-specific δ¹³C analytical method for amino acids and nucleotides using new LC-IRMS system and to clarify the relationship between intracellular carbon flow and molecular-level δ¹³C signatures. Standard amino acids and nucleotides were analyzed to evaluate accuracy and precision, and mixed-mode chromatographic conditions were optimized. Proteins were hydrolyzed by acid treatment and nucleic acids by enzymatic digestion with nuclease P1 prior to LC-IRMS analysis. The method demonstrated high analytical performance and effective separation of 16 amino acids and 9 nucleotides, confirming its applicability to biological samples.

---