Poster Presentations
Day 2, June 11（Thu.） Room P (5F 501+502)
- 2P-16
An Integrative Proteo-metabolomic Analysis for Reversing Splenic Immunosenescence
(DGIST)
oRafiqul Alam, Junhee Kim, Min-Sik Kim
Aging is associated with progressive decline in immune function, known as immunosenescence, with the spleen playing a critical role in regulating systemic immune responses. However, the molecular mechanisms underlying splenic aging and the potential for pharmacological reversal remain poorly understood. In this study, we aim to investigate whether rapamycin can modulate age-associated molecular changes in the spleen. To do so, an integrated proteomic and metabolomic was carried out using young (2-month-old) and aged (18-month-old) C57BL/6J mice treated with rapamycin. A group of aged mice received rapamycin treatment to evaluate its potential to reverse aging-associated molecular signatures. Following treatment, spleen tissues were collected and prepared for comprehensive proteomic and metabolomic profiling. High-resolution liquid chromatography–mass spectrometry (LC–MS) was used to characterize global protein and metabolite changes associated with aging and rapamycin intervention. The ongoing analysis is expected to provide insights into metabolic and proteomic pathways involved in splenic immunosenescence and reveal molecular signatures associated with rapamycin-mediated tissue recovery. This study highlights the potential of mass spectrometry–based multi-omics approaches to better understand immune aging and identify targets for therapeutic intervention.