Program

Poster Presentations

Day 2, June 11（Thu.）　　Room P (5F 501+502)

2P-15

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Exploring Biomarkers for Pediatric Epilepsy through Lipid Profiling of Extracellular Vesicles from Cerebrospinal Fluid

(¹Teikyo Univ., ²Tohoku Univ., ³Tohoku Univ., ⁴Okayama Univ., ⁵Tohoku Univ.)
^oArisa Ishii¹, Yuko Iwasaki², Tomoko Fukuuchi¹, Noriko Yamaoka¹, Kuniyasu Niizuma^2,3, Tomoyuki Akiyama⁴, Daisuke Saigusa^1,5

Epilepsy is a chronic neurological disorder characterized by recurrent seizures, affecting over 50 million people worldwide, with a higher incidence in infants and the elderly. Diagnosis relies mainly on electroencephalography (EEG); however, single EEG recordings are often insufficient in pediatric patients due to noise and limited cooperation. Extracellular vesicles (EVs) are 30–150 nm lipid bilayer structures involved in intercellular communication and are abundant in cerebrospinal fluid, predominantly derived from neural cells. We analyzed the lipidome of EVs isolated from cerebrospinal fluid of pediatric patients with epilepsy (n = 14) and patients without epilepsy (n = 16) to identify potential biomarkers for epilepsy onset. EVs were isolated using the MagCapture^TM Exosome Isolation Kit PS Ver.2, and lipids were extracted via the Bligh & Dyer method. Lipid species were separated by UHPLC-FTMS and identified using LipidSearch, followed by statistical analysis with MetaboAnalyst 6.0. Over 3,000 lipid species were detected. Compared with patients without epilepsy, ten lipid species including phosphatidylcholines were significantly increased, while 21 species including dihydroceramides and diacylglycerols were significantly decreased (p < 0.05). Alterations in short-chain ceramide balance may serve as potential predictive biomarkers. Further comprehensive analysis of short-chain ceramides is ongoing.

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