Poster Presentations
Day 2, June 11（Thu.） Room P (5F 501+502)
- 2P-14
Development of a Detection Method for the HIF-PH Inhibitor Roxadustat Using Extracellular Vesicles from Dried Blood Spots
(1Teikyo Univ., 2Teikyo Univ., 3Tohoku Univ., 4Tohoku Univ.)
oTaiki Hirai1, Arisa Ishii2, Tomoko Fukuuchi1, Noriko Yamaoka1, Norio Suzuki3,4, Daisuke Saigusa1,4
The use of substances classified as peptide hormones, growth factors, related substances, and mimetics (S2) by the World Anti-Doping Agency (WADA) has increased in sports doping. In particular, hypoxia-inducible factor prolyl hydroxylase (HIF-PH) inhibitors are difficult to detect using conventional urine-based doping tests because of their rapid metabolism and low urinary excretion. Therefore, the development of new detection methods for HIF-PH inhibitors is required. Extracellular vesicles (EVs) are lipid bilayer vesicles (30–150 nm) derived from endosomes and are known to contain various biomolecules, including proteins, nucleic acids, and small molecules. In this study, we investigated whether the HIF-PH inhibitor roxadustat could be detected from EVs derived from dried blood spots (DBS). EVs were isolated from DBS prepared from roxadustat-administered mice and analyzed by UHPLC–MS/MS. Roxadustat was successfully detected in DBS-derived blood EVs. These results suggest that EVs in DBS may serve as a potential target for detecting HIF-PH inhibitors in anti-doping testing.