Poster Presentations
Day 2, June 11（Thu.） Room P (5F 501+502)
- 2P-07（3C-P2-1516）
Comparative Proteomic Analysis of Molecular Distribution and Environmental Responsiveness in Mesenchymal Stromal Cell-Derived Extracellular Vesicles
(Yokohama City Univ.)
oYuzuka Kuba, Tohru Sugawara, Nana Kawasaki
Mesenchymal stromal cells (MSCs) promote tissue repair and immunomodulation and are promising therapeutic candidates for skin injury and graft-versus-host disease. Their therapeutic effects are thought to depend largely on the secretome, particularly extracellular vesicles (EVs). However, the molecular relationship between MSCs and MSC-EVs, and their responses to environmental stress, remain unclear. In this study, human bone marrow MSCs from three donors were cultured under normoxic (20% O₂, +glucose) and pseudo-ischemic (1% O₂, −glucose) conditions. Cellular, EV, and supernatant fractions were analyzed using DIA-based quantitative proteomics. Under pseudo-ischemic conditions, protein profiles showed low correlation between cells and EVs, indicating that EV content does not simply reflect intracellular protein abundance. EVs showed partial similarity to the supernatant fraction. When culture conditions changed, the cellular proteome remained relatively stable, whereas the EV proteome showed greater variability, with little correlation in expression changes between cells and EVs. Gene ontology analysis revealed enrichment of energy metabolism and cytoskeletal pathways in cells, and cell adhesion and extracellular matrix–related pathways in EVs. These findings suggest that MSC-EVs may not simply mirror the intracellular state of MSCs and display distinct molecular responses to environmental stress.