Proposed Sessions
Day 2, June 11（Thu.） 10:30-10:45 Room D (5F 511+512)
- 2D-P1-1030
TOF-SIMS Imaging of Whole Lipids in Biological Tissue with Enhanced Ionization by Ionic Liquid Matrix
(ULVAC-PHI)
oItsuko Ishizaki, Noriaki Sanada
Mass spectrometry Imaging (MSI) is a powerful technique for visualizing the spatial distribution of chemical species in biological samples. Time-of-flight secondary ion mass spectrometry (TOF-SIMS) enables imaging with submicron spatial resolution and the detection of a broad range of molecular ions, including intact biological molecules. However, the observation of high-mass components remains challenging due to limitations in ionization efficiency and sample degradation.
In this study, we present a novel sample preparation method for TOF-SIMS using an ionic liquid (IL) matrix to enhance the ionization efficiency of high-mass lipid ions. This approach increases the ionization efficiency of biological molecules by having an appropriate distribution of ionic liquid into the sample. By reducing fragmentation, this method makes it possible to exceed the static limit and increases the lipid ion signal by nearly three orders of magnitude. Furthermore, it enables stable molecules and ion-induced secondary electrons in cellular structures without compromising spatial resolution. This technique enables molecular imaging at the highest spatial resolution achievable by TOF-SIMS, expanding the possibilities for molecular observation. These advancements have significant opportunities to improve TOF-SIMS-based MSI applications, offering new opportunities for lipidomics and biological research.