Proposed Sessions
Day 2, June 11（Thu.） 10:15-10:30 Room D (5F 511+512)
- 2D-P1-1015
Development of novel on-tissue chemical derivatization reagents targeting carboxylic acids for MALDI-MS imaging
(1Shimadzu, 2Kobe Univ., 3Kwansei Gakuin Univ.)
oKaoru Nakagawa1, Akiko Kubo2, Kei Murakami3, Yota Sakakibara3, Masaharu Toguchi3, Yayoi Ichiki1, Manami Kobayashi1, Junichi Masuda1
MS imaging (MSI) combines molecular and spatial information to map chemical distributions in tissues and cells, and MALDI is widely used for MSI due to its accessibility and improved spatial resolution and throughput. However, MSI performance can be limited by ion suppression, low analyte abundance, poor ionization efficiency, and interferences from endogenous compounds or sample-preparation procedures, which can hinder detection of certain molecular species. Chemical derivatization (CD) addresses these issues by adding functional groups or pre-charged tags to enhance ionization. In particular, mass shifting and permanent-charge introduction can both increase ionization efficiency and separate analytes from isobaric interferences, improving MSI sensitivity and selectivity. In this study, an on tissue chemical derivatization (OTCD) workflow for carboxylic acids in the murine cecum was developed, a novel quaternary ammonium salt CD reagent was synthesized, and OTCD conditions were optimized to enable improved visualization of carboxylic-acid–containing metabolites.