Proposed Sessions
Day 2, June 11（Thu.） 9:45-10:00 Room D (5F 511+512)
- 2D-P1-0945
Analytical Complementarity of LC–MS and MS Imaging in Metabolomic Analysis of Non-Model Animals
(1OIST, 2UOsaka, 3Miruion)
oYayoi Hongo1, Shuichi Shimma2,3, Hiromi Saito2, Hiroshi Watanabe1
To promote multi-omics integration in non-model animals, we evaluated the analytical complementarity of LC–MS and mass spectrometry imaging (MSI) in the cnidarian N. vectensis. LC–MS analysis of adult polyps detected 2-hydroxytryptamine, whereas its structural isomer serotonin, previously suggested by immunostaining, was not observed. Although LC–MS enables isomer discrimination, extraction-based analysis results in the loss of spatial information, limiting interpretation of tissue-specific distribution. MSI was therefore applied to the same specimen system to visualize metabolite localization. Because 2-hydroxytryptamine and serotonin share identical precursor m/z values and overlapping product ions in MS/MS, MSI alone cannot distinguish these isomers. However, MSI revealed that aromatic amino acid decarboxylation products, which appeared uniformly distributed by LC–MS, were in fact localized to the polyp body surface.These results demonstrate that combining LC–MS-based structural discrimination with MSI-based spatial resolution provides complementary analytical information not attainable by either method alone.