Symposium Sessions
Day 2, June 11（Thu.） 9:15-9:40 Room C (4F 413)
- 2C-S1-0915
Direct and inverse stable isotope labeling for catabolic analysis of plant specialized metabolites
(1Chiba Univ., 2RIKEN CSRS)
oRyosuke Sugiyama1,2
Stable isotope tracer experiments have been a powerful strategy to reveal the metabolic flow in living organisms, especially in combination with mass spectrometry-based metabolomics. Recently, we clarified that endogenous storage of glucosinolates, a class of sulfur-rich plant metabolites, is exploited as a sulfur source in a model plant, Arabidopsis thaliana (Sugiyama et al., PNAS 2021). HPLC–MS/MS analysis coupled with 34S- or deuterium-labeled glucosinolates depicted the retrograde sulfur flow connecting glucosinolates to primary metabolites such as Cys at the atomic level. These findings demonstrate the bidirectional interaction between primary and specialized metabolism. However, it remains challenging to efficiently introduce stable isotopes into specialized metabolites with complex structures as feeding source compounds.
In this presentation, I demonstrate the integrated analysis of direct and ‘inverse’ stable isotope labeling coupled with LC–MS and ICP–MS to comprehend the catabolic pathway of selected plant specialized metabolites toward primary metabolites.