Luncheon Seminar
Day 2, June 11（Thu.） 12:00-13:00 Room C (4F 413)
- 2C-L-1200
Charge Detection Mass Spectrometry of Protein Complexes: from Hundreds of kDa to the Mega-Dalton Scale
(UOsaka)
oYuki Yamaguchi, Susumu Uchiyama
Native mass spectrometry (Native-MS) is a well-established technique for the direct mass analysis of protein complexes, but accurate mass determination remains challenging for very large or highly heterogeneous species because charge-state peaks cannot be resolved. Charge detection mass spectrometry (CDMS) addresses this limitation by independently measuring the charge and m/z of individual ions, enabling mass determination of heterogeneous protein complexes from accumulated single-ion measurements. Here, we present applications of ELIT-based CDMS on the Xevo™ CDMS platform to proteins and protein complexes. Charge-state peaks were resolved even for proteins up to 500 kDa, such as IgG and β-galactosidase, enabling Native-MS-like analysis. We also demonstrate the determination of mass distributions for highly heterogeneous glycoproteins, recombinant adeno-associated viruses (rAAVs), and an ultralarge ~8 MDa complex. For rAAVs, particle masses were determined within 1% of theoretical values, enabling assessment of full-to-empty ratios, packaged genome size, and capsid protein composition. These results highlight CDMS as a powerful mass measurement technique for the analysis of large and heterogeneous protein complexes.