Program

Symposium Sessions

Day 2, June 11（Thu.）　9:35-9:55　Room B (4F 411+412)

2B-S1-0935

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Comparative Evaluation of LC/MS Approaches for Integrated Human Plasma Metabolomics

(¹UOsaka, ²Kyushu Univ., ³Kyushu Univ., ⁴Nippon Sanso, ⁵AIST, ⁶Niigata Univ., ⁷SAIL Technologies, ⁸Keio Univ., ⁹Kyushu Univ., ¹⁰UOsaka)
^oMasatomo Takahashi^1,2,3, Yuri Imado³, Akari Ikeda⁴, Yuki Soma⁵, Shunsuke Aburaya², Kohta Nakatani⁶, Tsutomu Terauchi⁷, Takayoshi Matsuda⁷, Akiyoshi Hirayama⁸, Taizo Hanai⁹, Takeshi Bamba^2,3,10, Yoshihiro Izumi¹

Information on candidate biomarker metabolites is expected to play a key role in personalized and precision medicine. Although LC/MS offers broad coverage and high sensitivity for metabolomics, its suitability for identifying and quantifying hydrophilic metabolites remains debated. In this study, we evaluated four LC/MS approaches—HILIC, AEX-IC, PFPP-RPLC, and unified-HILIC/AEX—coupled to the same Orbitrap mass spectrometer, aiming to facilitate integration of human plasma metabolome data. Qualitative performance was assessed using 511 hydrophilic metabolite standards and NIST SRM 1950, focusing on coverage, peak width, sensitivity, and isomer separation. Quantitative performance for 63 metabolites in SRM 1950 was evaluated using a stable isotope-labeled internal standard (SILIS) mixture derived from Escherichia coli. We also estimated concentration values for 29 metabolites lacking certified values. Our results demonstrate that absolute quantification using SILIS enables effective integration of hydrophilic metabolite data across LC/MS platforms.

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