Symposium Sessions
Day 2, June 11（Thu.） 9:15-9:35 Room B (4F 411+412)
- 2B-S1-0915
Reproducibility of Blood Lipidomics and Proteomics in a Large-Scale Japanese Cohort
(Univ. Tokyo)
oSuzumi Tokuoka, Yoshiya Oda
Background: Aging and chronic liver diseases (MASLD/MASH/HCV) involve complex inflammation and metabolic dysfunction. To address reproducibility challenges across facilities, this study identified robust molecular patterns using targeted lipidomics and proteomics in approximately 4,000 Japanese serum/plasma samples across five independent cohorts.
Methods: Approximately 4,000 serum/plasma samples from community-dwelling older adults and patients with liver disease were analyzed; 618 of these were plasma samples from the liver-disease cohort. Lipids (~500 species) were measured via LC-MS/MS, and proteins via Olink® PEA (Target 96/48 platforms).
Results: Across all cohorts, CXCL9, CCL11, and specific phosphatidylcholines (PC 31:0, 32:0) positively correlated with aging, while lysophosphatidylcholines (LPC-LA, LPC-AA) consistently showed negative correlations, regardless of matrix or quantification method. In MASH, changes in seven proteins (e.g., CASP-8, CCL20) and specific lipid shifts—decreased ether-PE and increased saturated SM—were reproduced across three cohorts. FDR correction ensured the selection of only consistently validated molecules.
Conclusion: This study establishes a highly robust blood molecular reference dataset for the Japanese population. These patterns, independent of matrix or measurement conditions, provide a foundation for understanding pathologies and future risk stratification. Furthermore, this research demonstrates the high reliability of our integrated dual-omics approach for generating high-quality data.