Poster Presentations
Day 1, June 10（Wed.） Room P (5F 501+502)
- 1P-47
Implementation of microscale chromatography for high sensitivity & throughput quantification of peptide-based GLP-1 receptor agonists and oligonucleotide drugs in plasma
(1Nihon Waters, 2Waters)
oEtsuko Suzuki1, Robert Plumb2, Mary Trudeau2
Capillary UHPLC – tandem quadrupole mass spectrometry has been demonstrated to increase sensitivity and reduce sample volume requirements in drug discovery and development DMPK studies. Thus, facilitating both the replace, reduce, refine (3Rs) principles minimizing animals use in research and patient centric sampling concept. Here we present the use of a high throughput capillary LC tandem quadrupole MS methodology for the quantification of peptide-based GLP-1 receptor agonists in human plasma. The system demonstrated a 10- fold increase in sensitivity over analytical scale LC-MS/MS, delivering LLOQ in the 20 pg/mL range, with 4- orders of magnitude linearity and the robustness and reproducibility meeting FDA M10 guidelines. A similar increase in sensitivity was observed for the analysis of antisense oligonucleotides in plasma.